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ribotm fluorescent in situ hybridisation kit  (Ribobio co)

 
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    Structured Review

    Ribobio co ribotm fluorescent in situ hybridisation kit
    Ribotm Fluorescent In Situ Hybridisation Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+in+situ+hybridisation+kit/ribotm+fluorescent+in+situ+hybridization+kit/pm40436288-112-16-7
    Average 90 stars, based on 1 article reviews
    ribotm fluorescent in situ hybridisation kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.
    Article Snippet: .. The fluorescence in situ hybridisation (FISH) assay was performed using a Fluorescence in Situ Hybridisation Kit (Ribobio, Guangzhou, China) according to the manufacturer's instructions. .. After being fixed in 4% paraformaldehyde (Solarbio), PDLSCs were washed with phosphatebuffered saline (PBS) containing 0.5% Triton X-100 (Solarbio) to increase cell permeability.

    Article Title: circASAP1 induces renal clear cell carcinoma ferroptosis by binding to HNRNPC and thereby regulating GPX4.
    Article Snippet: The circASAP1 oligonucleotide probe with Cy3 fluorescent labeling was synthesized by RiboBio, China. .. The probe was hybridised into cells using a fluorescence in situ hybridisation kit (RiboBio, China). .. Images were taken by a confocal laser scanning microscope (FV1000, Olympus, Japan).

    In Situ:

    Article Title: Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.
    Article Snippet: .. The fluorescence in situ hybridisation (FISH) assay was performed using a Fluorescence in Situ Hybridisation Kit (Ribobio, Guangzhou, China) according to the manufacturer's instructions. .. After being fixed in 4% paraformaldehyde (Solarbio), PDLSCs were washed with phosphatebuffered saline (PBS) containing 0.5% Triton X-100 (Solarbio) to increase cell permeability.

    Article Title: circASAP1 induces renal clear cell carcinoma ferroptosis by binding to HNRNPC and thereby regulating GPX4.
    Article Snippet: The circASAP1 oligonucleotide probe with Cy3 fluorescent labeling was synthesized by RiboBio, China. .. The probe was hybridised into cells using a fluorescence in situ hybridisation kit (RiboBio, China). .. Images were taken by a confocal laser scanning microscope (FV1000, Olympus, Japan).

    Hybridization:

    Article Title: Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.
    Article Snippet: .. The fluorescence in situ hybridisation (FISH) assay was performed using a Fluorescence in Situ Hybridisation Kit (Ribobio, Guangzhou, China) according to the manufacturer's instructions. .. After being fixed in 4% paraformaldehyde (Solarbio), PDLSCs were washed with phosphatebuffered saline (PBS) containing 0.5% Triton X-100 (Solarbio) to increase cell permeability.

    Article Title: circASAP1 induces renal clear cell carcinoma ferroptosis by binding to HNRNPC and thereby regulating GPX4.
    Article Snippet: The circASAP1 oligonucleotide probe with Cy3 fluorescent labeling was synthesized by RiboBio, China. .. The probe was hybridised into cells using a fluorescence in situ hybridisation kit (RiboBio, China). .. Images were taken by a confocal laser scanning microscope (FV1000, Olympus, Japan).

    Fluorescence In Situ Hybridization:

    Article Title: Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.
    Article Snippet: .. The fluorescence in situ hybridisation (FISH) assay was performed using a Fluorescence in Situ Hybridisation Kit (Ribobio, Guangzhou, China) according to the manufacturer's instructions. .. After being fixed in 4% paraformaldehyde (Solarbio), PDLSCs were washed with phosphatebuffered saline (PBS) containing 0.5% Triton X-100 (Solarbio) to increase cell permeability.

    other:

    Article Title: circRNA_0005529 facilitates growth and metastasis of gastric cancer via regulating miR-527/Sp1 axis.
    Article Snippet: A fluorescence in situ hybridisation (FISH) kit (RiboBio) was used to perform FISH.



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    Binding of IFIH1‐binding RNA 1 (IBR1) to IFIH1. (A) RNA fluorescence in situ <t>hybridisation</t> (FISH) and immunofluorescence (IF) experiments revealed that IBR1 co‐localised with IFIH1 in bone marrow‐derived macrophages (BMDMs). Scale bar: 25 µm. (B) RNA immunoprecipitation (RIP)‒PCR detection of the enrichment of IFIH1 on IBR1 in BMDMs. Immunoglobulin G (IgG) was used as the negative control. (C) Electrophoretic mobility shift assays (EMSAs) revealed that IBR1 binds to IFIH1 in vitro. (D and E) Silver staining of gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‒PAGE) and Western blotting revealed that IFIH1 was enriched, as determined by IBR1 pull‐down assays.
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    Binding of IFIH1‐binding RNA 1 (IBR1) to IFIH1. (A) RNA fluorescence in situ <t>hybridisation</t> (FISH) and immunofluorescence (IF) experiments revealed that IBR1 co‐localised with IFIH1 in bone marrow‐derived macrophages (BMDMs). Scale bar: 25 µm. (B) RNA immunoprecipitation (RIP)‒PCR detection of the enrichment of IFIH1 on IBR1 in BMDMs. Immunoglobulin G (IgG) was used as the negative control. (C) Electrophoretic mobility shift assays (EMSAs) revealed that IBR1 binds to IFIH1 in vitro. (D and E) Silver staining of gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‒PAGE) and Western blotting revealed that IFIH1 was enriched, as determined by IBR1 pull‐down assays.
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    Binding of IFIH1‐binding RNA 1 (IBR1) to IFIH1. (A) RNA fluorescence in situ <t>hybridisation</t> (FISH) and immunofluorescence (IF) experiments revealed that IBR1 co‐localised with IFIH1 in bone marrow‐derived macrophages (BMDMs). Scale bar: 25 µm. (B) RNA immunoprecipitation (RIP)‒PCR detection of the enrichment of IFIH1 on IBR1 in BMDMs. Immunoglobulin G (IgG) was used as the negative control. (C) Electrophoretic mobility shift assays (EMSAs) revealed that IBR1 binds to IFIH1 in vitro. (D and E) Silver staining of gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‒PAGE) and Western blotting revealed that IFIH1 was enriched, as determined by IBR1 pull‐down assays.
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    Image Search Results


    Binding of IFIH1‐binding RNA 1 (IBR1) to IFIH1. (A) RNA fluorescence in situ hybridisation (FISH) and immunofluorescence (IF) experiments revealed that IBR1 co‐localised with IFIH1 in bone marrow‐derived macrophages (BMDMs). Scale bar: 25 µm. (B) RNA immunoprecipitation (RIP)‒PCR detection of the enrichment of IFIH1 on IBR1 in BMDMs. Immunoglobulin G (IgG) was used as the negative control. (C) Electrophoretic mobility shift assays (EMSAs) revealed that IBR1 binds to IFIH1 in vitro. (D and E) Silver staining of gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‒PAGE) and Western blotting revealed that IFIH1 was enriched, as determined by IBR1 pull‐down assays.

    Journal: Clinical and Translational Medicine

    Article Title: IBR1, a novel endogenous IFIH1‐binding dsRNA, governs IFIH1 activation and M1 macrophage polarisation in ARDS

    doi: 10.1002/ctm2.70027

    Figure Lengend Snippet: Binding of IFIH1‐binding RNA 1 (IBR1) to IFIH1. (A) RNA fluorescence in situ hybridisation (FISH) and immunofluorescence (IF) experiments revealed that IBR1 co‐localised with IFIH1 in bone marrow‐derived macrophages (BMDMs). Scale bar: 25 µm. (B) RNA immunoprecipitation (RIP)‒PCR detection of the enrichment of IFIH1 on IBR1 in BMDMs. Immunoglobulin G (IgG) was used as the negative control. (C) Electrophoretic mobility shift assays (EMSAs) revealed that IBR1 binds to IFIH1 in vitro. (D and E) Silver staining of gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‒PAGE) and Western blotting revealed that IFIH1 was enriched, as determined by IBR1 pull‐down assays.

    Article Snippet: Fluorescence in situ hybridisation (FISH) was conducted on BMDMs or CD14 + monocytes utilising the Ribo Fluorescent In Situ Hybridisation Kit from Ribo Bio.

    Techniques: Binding Assay, Fluorescence, In Situ, Hybridization, Immunofluorescence, Derivative Assay, RNA Immunoprecipitation, Negative Control, Electrophoretic Mobility Shift Assay, In Vitro, Silver Staining, Polyacrylamide Gel Electrophoresis, Western Blot